Data/hora: 20/05/2024 05:35:21

Provedor de dados: 	5	
País: 	France	
Título: 	Effect of oxygen and temperature on the dynamic of the dominant bacterial populations of pig manure and on the persistence of pig-associated genetic markers, assessed in river water microcosms	
Autores: 	Marti, R.; Mieszkin, Sophie; Solecki, O.; Pourcher, A. -m.; Hervio-heath, Dominique; Gourmelon, Michele.	
Data: 	2011-11	
Ano: 	2011	
Palavras-chave: 	Lactobacillus amylovorus; Microbial source tracking; Oxygen; Persistence; Pig-associated Bacteroidales markers; Quantitative real-time PCR; River water; Temperature.	
Resumo: 	Aims: The aim is to evaluate the dynamic of Bacteroides-Prevotella and Bacillus-Streptococcus-Lactobacillus populations originating from pig manure and the persistence of pig-associated markers belonging to these groups according to temperature and oxygen. Methods and Results: River water was inoculated with pig manure and incubated under microaerophilic and aerobic conditions, at 4 and 20 degrees C over 43 days. The diversity of bacterial populations was analysed by capillary electrophoresis-single-strand conformation polymorphism. The persistence of the pig-associated markers was measured by real-time PCR and compared with the survival of Escherichia coli and enterococci. Decay was characterized by the estimation of the time needed to produce a 1-log reduction (T90). The greatest changes were observed at 20 degrees C under aerobic conditions, leading to a reduction in the diversity of the bacterial populations and in the concentrations of the Pig-1-Bac, Pig-2-Bac and Lactobacillus amylovorus markers with a T90 of 10 5, 8 1 and 17 2 days, respectively. Conclusions: Oxygen and temperature were found to have a combined effect on the persistence of the pig-associated markers in river waters. Significance and Impact of the Study: The persistence profiles of the Pig-1-Bac, Pig-2-Bac and Lact. amylovorus markers in addition to their high specificity and sensitivity support their use as relevant markers to identify pig faecal contamination in river waters.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00053/16470/14534.pdf

DOI:10.1111/j.1365-2672.2011.05131.x	
Editor: 	Wiley-blackwell	
Relação: 	http://archimer.ifremer.fr/doc/00053/16470/	
Formato: 	application/pdf	
Fonte: 	Journal Of Applied Microbiology (1364-5072) (Wiley-blackwell), 2011-11 , Vol. 111 , N. 5 , P. 1159-1175	
Fonte: 	2011 The Authors. Journal of Applied Microbiology 2011 The Society for Applied Microbiology